What can I use other than beta-mercaptoethanol to reduce my mAb and/or other proteins? - WKB201200
Article number: 201200
ENVIRONMENT
- Protein
- mAb
- Monoclonal Antibody
- IgG
- Reduce
- Disulfide Bonds
ANSWER
DTT (dithiothreitol)
ADDITIONAL INFORMATION
Dithiothreitol (DTT) can be used to reduce mAbs and proteins.
Note: 20 min at 60 °C is typical, but if you increase temperature, you can reduce incubation time (for example, 15 min at 80 °C).
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