With an ACQUITY Arc, there are no peaks in the UV chromatograms - WKB196116

SYMPTOMS

• There is no peak in the chromatogram of the vial that was injected last in the sample set
• The sample volume in the vial for which no peak is detected is about 200 uL
• A 2-mL TruView LCMS Certified vial is used

ENVIRONMENT

• ACQUITY Arc
• ACQUITY UPLC Tunable UV Detector
• 2489 UV/Vis Detector

CAUSE

The sample volume in the vial is less than the minimum residual amount.

FIX or WORKAROUND

When using TruView LCMS Certified 2-mL vial with ACQUITY Arc, the minimum residual volume is 360 µL; therefore, the sample volume to prepare for the vial is as follows:

• Required sample volume = Minimum residual volume (360 µL) + Injection volume

The minimum residual volume is the value below which the sample can no longer be drawn. If possible, add a little more sample to the required sample volume noted above.

• Alternatively, change the vial to a Maximum Recovery Vial or a Total Recovery Vial.

ADDITIONAL INFORMATION

Note the residual volume of the various vials listed at https://www.waters.com/waters/library.htm?cid=10000711&lid=10010954&locale=101.