What is the optimized cleaning procedure for difluoroacetic acid (DFA)? - WKB109893
ENVIRONMENT
- Mobile-phase additives
- IonHance difluoroacetic acid (DFA)
- LC
- LC-MS
- Solvents
ANSWER
Optimized cleaning protocol for DFA
Prime solution: 5% FA in 25:25:25:25 water/MeCN/MeOH/IPA
Wash solution: 2% FA in 25:25:25:25 water/MeCN/MeOH/IPA
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Remove sinkers from lines and switch to 5% prime solution using an “air gap” + “inbetween” solvent changing technique (original MP > air gap > inbetween vessel > air gap > wash solution). Be sure to wipe away any residual DFA solution before submerging into the 5% prime solution.
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Prime all lines in contact with DFA for 20 minutes with 5% prime solution.
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After priming, switch to 2% wash solution using an “air gap” + “inbetween” solvent changing technique; be sure to wipe away any residual 5% prime solution before submerging into 2% wash solution.
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Run 2% wash solution at 0.5 mL/min for a minimum of 1 hour to waste. Do not run high % FA through %S detector.
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Run series of intact mAb on Bioresolve RP column to check for signal intensity and adduct levels. Check for continuously increasing signal intensity.
ADDITIONAL INFORMATION
id109893, isopropanol, methanol, monoclonal antibody, XEVOQTOF