Can I run Atlantis PREMIER BEH C18 AX VanGuard FIT Column, 2.5 µm, 4.6 x 150 mm, 1/pk (186009385) on a traditional HPLC with a maximum pressure limit of 4,000 psi? - WKB97656

ENVIRONMENT

• High Performance Liquid Chromatography (HPLC)
• Ultra High Performance Liquid Chromatography (UHPLC)
• Column
• Bridged Ethylene Hybrid (BEH)
• eXtended Performance (XP) 2.5 um
• Polar compound retention
• Reversed-Phase
• Mixed-Mode
• Anion Exchange
• PREMIER
• High Performance Surfaces (HPS)
• MaxPeak HPS
• Fully Integrated Technology (FIT)
• VanGuard

ANSWER

Yes.

ADDITIONAL INFORMATION

To use on a traditional HPLC system, the organic modifier should be acetonitrile and not methanol. 

Use of methanol causes a significant increase in column back pressure as the methanol and water mix. 

Use of acetonitrile helps keep pressures lower. 

Heating the column also reduces back pressure.

You can estimate back pressures by using the Columns Calculator Version 2.0. (You may need approval from your IT department to download this calculator since it is an executable file).

Atlantis PREMIER Columns Infographic

Atlantis Premier BEH C18 AX Columns Care and Use Manual

A New Mixed-Mode Reversed-Phase/Anion-Exchange Stationary Phase Based on Hybrid Particles

VanGuard FIT: A Breakthrough in Guard Column Performance for Challenging Chromatographic Separations