Which Ultrahydrogel columns does Waters recommend for analyzing polysaccharides with a molecular weight range from 80 kDa down to as low as 100 Da? - WKB97632

ENVIRONMENT

• High Performance Liquid Chromatography (HPLC)
• Gel Permeation Chromatography (GPC)
• Size Exclusion Chromatography (SEC)
• Gel Filtration Chromatography (GFC)
• Column
• Polysaccharides
• Water-Soluble Polymers

ANSWER

Install the guard, and then the following columns from largest pore size to smallest pore size:

• WAT011565 - Ultrahydrogel Guard Column, 125Å, 6 µm, 6 mm x 40 mm, 1/pk
• WAT011530 - Ultrahydrogel Column, 500Å, 10 µm, 7.8 mm x 300 mm, 5K - 400K, 1/pk
• WAT011525 - Ultrahydrogel Column, 250Å, 6 µm, 7.8 mm x 300 mm, 1K - 80K, 1/pk
• WAT011520 - Ultrahydrogel Column, 120Å, 6 µm, 7.8 mm x 300 mm, 100 - 5K, 1/pk

ADDITIONAL INFORMATION

We recommend the 500A column because the molecular weight distribution of the polysaccharides could be higher than 80 kDa.

Waters Gel Permeation Chromatography (GPC) Capabilities Brochure

Ultrahydrogel Columns Care & Use Manual