What is a good column choice for analysis of 4-aminopyridine (4-AP)? - WKB94222
Article number: 94222
ENVIRONMENT
- Bridged ethylene hybrid (BEH)
- Hydrophilic interaction chromatography (HILIC)
- XBridge
- CORTECS
ANSWER
Reversed-Phase
XBridge BEH C18
HILIC
CORTECS HILIC
XBridge BEH HILIC
XBridge BEH Amide
ADDITIONAL INFORMATION
Waters has no application notes for the analysis of this compound.
Experimentation is needed to verify that these column choices work for the intended purpose.
Reversed-phase: For best retention, use a mobile phase that has a pH 2 pH units higher than the pKa of the analyte. In reversed-phase, the analyte should retain better when neutral.
HILIC: For best retention, use a mobile phase that has a pH at least 2 pH units lower than the pKa of the analyte. The pH should be midrange, so that silanol groups on the particle will be ionized, making the packing more polar. In HILIC, the analyte should retain better when ionized.
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