How does reverse-phase chromatography separate compounds? - WKB86226
Article number: 86226
ENVIRONMENT
- Reverse-phase chromatography
ANSWER
Reverse-phase chromatography separates compounds by how polar and nonpolar they are in relation to each other.
ADDITIONAL INFORMATION
A reverse-phase column surface is nonpolar, and nonpolar compounds are more attracted to the nonpolar surface in the presence of water and organic mobile phase.
For additional information, see the Beginners Guide to Liquid Chromatography.
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