How many injections can I expect to get on an ACQUITY BEH SEC column? - WKB86119

ENVIRONMENT

• ACQUITY BEH SEC column
• Bridged ethylene hybrid (BEH)
• Size exclusion chromatography (SEC)

ANSWER

Column lifetime for size exclusion-based separation columns is critically dependent on the cleanliness of the mobile phases and samples.

The number of injections could be as much as 900 (see Improving the Lifetime of UPLC SEC Columns Using Short Guard Columns) when using guard columns along with implementation of proper mobile phase handling procedures.

Column lifetimes suffer when improper mobile phase and/or sample preparation practices are used.

ADDITIONAL INFORMATION

Also see:

Size-Exclusion Chromatography (SEC) Optimization Guide (PDF download page)

Size-Exclusion Chromatography (SEC) Optimization Guide (online guide)