What is the difference between the Atlantis dC18 and T3 columns? - WKB8555

ENVIRONMENT

• Atlantis dC18 column
• Atlantis T3 column

ANSWER

The dC18 was redesigned to improve column life at low pH. The second-generation column is called the Atlantis T3.

ADDITIONAL INFORMATION

Both of these are C18 columns, intended for use with 100% aqueous mobile phase for improved separation of polar analytes. The dC18 has a pH rangeof 3 to 7; the T3 has a pH range of 2 to 8.
The dC18 was originally designed for polar analytes. But over time, Waters scientists realized that column life at lower pH was not as good as they expected.
The T3 design intends to keep column performance as close as possible to the dC18. One change was the use of three bonds to attach the C18 ligand to the particle surface, which makes the T3 more stable at lower pHs. For most methods, the two columns should produce very similar chromatography. The T3 should have much greater column life at pH extremes, and it may have slightly better column life at neutral pHs.

Atlantis T3, dC18, and HILIC Silica Columns Care & Use Manual (736000640)