What is suggested to get better resolution of my peptides? - WKB82109
Article number: 82109
ENVIRONMENT
- Peptide Separation Technology (PST)
- Method development
- Resolution
- Gradient slope
- XBridge
- ACQUITY BEH
ANSWER
Be sure your gradient is set up so that it does not increase more than one column volume per 1% change in mobile phase conditions.
ADDITIONAL INFORMATION
You could also look at raising the temperature, going with a smaller particle size, using a longer column, changing the flow rate, or using a different chemistry.
For additional information, see Systematic Strategies for Developing Peptide Maps.