What is the recommended cleaning procedure for my Carbohydrate Analysis column? - WKB81741

ENVIRONMENT

• Column care and use
• Column cleaning
• Carbohydrate Analysis column

ANSWER

The column should be flushed with 80:20 acetonitrile/water or 75:25 acetonitrile/water to start for 50 minutes. After this initial flush, the column can be cleaned with 60:20:20 acetonitrile/methanol/water, 50:20:30 acetonitrile/methanol/water, or 60:40 acetonitrile/methanol.

ADDITIONAL INFORMATION

The column should be flushed with 20-25% water initially, to avoid precipitation of any sugar residues that might still be in the column. 

>50% methanol, IPA, or water increases the re-equilibration times after cleaning.

For additional information, see the High Performance Carbohydrate Column Care and Use Manual and What are the part numbers for the high performance carbohydrate column and guard column?