How should I store a uBondapak amino column WAT084040 for reverse phase applications? - WKB77538
Article number: 77538
ENVIRONMENT
- uBondapak
- micro Bondapak
- NH2
- Amino
- storage
- solvent
- care and use
- reverse phase
ANSWER
Flush with water, and then store in 70/30 ACN/H2O for reversed phase if column will be used within four days. For longer storage, use 100% acetonitrile.
ADDITIONAL INFORMATION
The NH2 chemistry lifetime is compromised in the presence of water. The amine ligand creates a basic environment inside the pores in the presence of water. These conditions hydrolyze the ligand from the silica particle as some silica is dissolved in the basic environment. The water also acts like a weak acid over time and further hydrolyzes the bonds that hold the amino ligand to the silica particle.
For additional information, see Section 24, page 31, Column Storage in the HPLC Troubleshooting Guide.
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