Does Waters have any application notes for lipid analysis by LCMS using normal phase? - WKB75977
ENVIRONMENT
- BEH HILIC
- Lipid analysis
ANSWER
Waters offers HILIC applications for LCMS.
See the application notes below.
UPLC BEH HILIC: The Preferred Separation Chemistry for Targeted Analysis of Phospholipids
Analysis of Intact Lipids from Biologics Matrices by UPLC/Ion Mobility TOF-MS
ADDITIONAL INFORMATION
Normal-phase UPLC separates lipid classes on the basis of their polar head group, whereas reversed-phase separates lipids according to their acyl chain length and number of double bonds. Hydrophilic interaction chromatography (HILIC) separation has been proposed as an alternative to normal-phase separation, offering better MS compatibility and using less toxic solvents. Recently, a two-dimensional separation using HILIC and reversed-phase has been proposed to maximize the separation of the lipidome before MS detection.
id75977,