What is a good column choice for the reversed-phase analysis of peptides? - WKB73119

ENVIRONMENT

• UltraPerformance Liquid Chromatography (UPLC)
• Ultra High Performance Liquid Chromatography (UHPLC)
• High Performance Liquid Chromatography (HPLC)
• Peptide separation technology (PST)
• Peptide mapping
• Peptide analysis
• Proteomics
• Column
• ACQUITY
• MaxPeak
• High Performance Surfaces (HPS)
• ACQUITY Premier
• Bridged Ethylene Hybrid (BEH)
• High Strength Silica (HSS)
• Charged Surface Hybrid (CSH)
• Sub-2um particle size
• XBridge
• XSelect

ANSWER

For UPLC

• ACQUITY Premier Peptide BEH C18, 130Å, 1.7 µm Columns**
• ACQUITY Premier Peptide BEH C18, 300Å, 1.7 µm Columns**
• ACQUITY Premier Peptide CSH C18 Column, 130Å, 1.7 µm Columns**
• ACQUITY Premier Peptide HSS T3 Column, 100Å, 1.8 µm Columns**
• ACQUITY UPLC Peptide BEH C18, 130Å, 1.7µm columns
• ACQUITY UPLC Peptide BEH C18, 300Å, 1.7µm columns
• ACQUITY UPLC Peptide CSH C18, 130Å, 1.7µm columns
• ACQUITY UPLC Peptide HSS T3, 100Å, 1.8µm columns

**MaxPeak HPS (High Performance Surfaces) column technology.

For UHPLC and HPLC:

• XBridge Premier Peptide BEH C18, 130Å, 2.5 µm Columns**
• XBridge Premier Peptide BEH C18, 300Å, 2.5 µm Columns**
• XSelect Premier Peptide CSH C18, 130Å, 2.5 µm Columns**
• XSelect Premier Peptide HSS T3, 100Å, 2.5 µm Columns**
• XBridge Peptide BEH C18, 130Å, 2.5µm XP, 3.5µm, 5µm, and 10µm Columns
• XBridge Peptide BEH C18, 300Å,  2.5µm XP, 3.5µm, 5µm, and 10µm Columns
• XSelect Peptide CSH C18, 130Å, 2.5µm XP, 3.5µm and 5µm Columns
• XSelect Peptide HSS T3, 100Å, 2.5µm XP, 5µm columns

**MaxPeak HPS (High Performance Surfaces) column technology.

 

ADDITIONAL INFORMATION

Peptides should not adsorb to the stainless steel frits or column body when using MaxPeak Premier columns.

BEH C18 is a good, general purpose, high ligand density C18 packing.

CSH C18 is a high ligand density C18 which also has positive surface charges in between the C18 ligands.  This reduces tailing of basic peptides when running in a low pH , low ionic strength mobile phase such as 0.1% formic acid.

HSS T3 is a low ligand density C18 which is designed for better retention of polar peptides.  This column can run in 100% aqueous mobile phase. 

MaxPeak Premier Columns Web Page

Peptide Reversed-Phase Columns Web Page

MaxPeak Premier Columns with MaxPeak High Performance Surfaces - Ordering Information

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