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How can I improve the resolution between two chromatographic peaks? - WKB71666

Article number: 71666

OBJECTIVE or GOAL

Improve the resolution between two chromatographic peaks.

ENVIRONMENT

  • Chromatography
  • Chromatographic resolution
  • Method development
  • Peak separation

PROCEDURE

  • Mechanically increase resolution potential by decreasing particle size.
  • Mechanically increase resolution potential by increasing column length.
  • Chemically change the resolution potential by adjusting the mobile phase composition.
  • Chemically change the resolution potential by changing to a different solvent in the mobile phase.
  • Chemically change the resolution potential by changing or adding a modifier, such as a buffer, to the mobile phase.
  • Change the selectivity of the separation and affect resolution by increasing or decreasing the column temperature.
  • Change the selectivity of the separation and affect resolution by changing the column chemistry.

ADDITIONAL INFORMATION

For additional information, see the Beginners Guide to Liquid Chromatography.

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