How can I improve the resolution between two chromatographic peaks? - WKB71666
Article number: 71666
OBJECTIVE or GOAL
Improve the resolution between two chromatographic peaks.
ENVIRONMENT
- Chromatography
- Chromatographic resolution
- Method development
- Peak separation
PROCEDURE
- Mechanically increase resolution potential by decreasing particle size.
- Mechanically increase resolution potential by increasing column length.
- Chemically change the resolution potential by adjusting the mobile phase composition.
- Chemically change the resolution potential by changing to a different solvent in the mobile phase.
- Chemically change the resolution potential by changing or adding a modifier, such as a buffer, to the mobile phase.
- Change the selectivity of the separation and affect resolution by increasing or decreasing the column temperature.
- Change the selectivity of the separation and affect resolution by changing the column chemistry.
ADDITIONAL INFORMATION
For additional information, see the Beginners Guide to Liquid Chromatography.
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