What is a good column choice for analysis of lactoferrin or other glycoproteins under denaturing conditions? - WKB70924
ENVIRONMENT
- HILIC
- BEH300 Amide
- Denaturing
- Protein analysis
- Glycoprotein
- Premier
ANSWER
ACQUITY PREMIER UPLC Glycoprotein BEH Amide (300A pore size) columns
ADDITIONAL INFORMATION
Under denaturing conditions, these columns are a great choice for this analysis.
This column is used as a HILIC (Hydrophilic interaction chromatography) column and is suitable for glycoproteins with molecular weights from 10 kDa up to 150 kDa.
0.1% TFA is used to neutralize the acidic and basic residues so that the primary retention mechanism is based on the glycans that are attached to the glycoprotein.
Developing High Resolution HILIC Separations of Intact Glycosylated Proteins
ACQUITY UPLC Glycoprotein BEH Amide, 300 Å, 1.7 μm Columns, ACQUITY Premier, 300 Å, 1.7 μm Columns, and Glycoprotein Performance Test Standard Care and Use Manual (lit code 720005408)
Premier Columns with MaxPeak High Performance Surfaces – Ordering Information (lit code 720007209EN)