Is it OK to backflush an ACQUITY UPLC HSS column? - WKB68689

ENVIRONMENT

• ACQUITY UPLC
• HSS
• column
• cleaning
• reverse
• backflush
• care and use

ANSWER

Yes, at a slow flow rate.

ADDITIONAL INFORMATION

When backflushing:

1. If you are using a guard column, disconnect it. 
2. Disconnect the column and install in the reverse direction. 
3. Do not connect the column to your detector.
4. Flow to waste.
5. Set flow rate to 0.1 mL/min for 2.1-mm ID columns and 0.3 mL/min for 3.0-mm ID columns.

See also: ACQUITY UPLC HSS Columns Care and Use Manual.