- start up
- initial conditions
- start procedure
- care and use
When a new CORTECS HILIC column is used, it must be flushed with 50/50 Acetonitrile:Water with 10mM of the buffer used in the method, for 50 column volumes.
After the 50/50 Acetonitrile:Water with 10mM buffer step, the column should be equilibrated for 20 column volumes in the initial conditions of a gradient or the isocratic mobile phase.
For additional information see CORTECS 2.7 um Columns Care and Use Manual