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What is the startup procedure for a CORTECS HILIC column? - WKB65943

Article number: 65943

ENVIRONMENT

  • column
  • CORTECS
  • HILIC
  • start up
  • initial conditions
  • start procedure
  • care and use

ANSWER

When a new CORTECS HILIC column is used, it must be flushed with 50/50 Acetonitrile:Water with 10mM of the buffer used in the method, for 50 column volumes.

After the 50/50 Acetonitrile:Water with 10mM buffer step, the column should be equilibrated for 20 column volumes in the initial conditions of a gradient or the isocratic mobile phase.

ADDITIONAL INFORMATION

For additional information see CORTECS 2.7 um Columns Care and Use Manual

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