What are the expected back pressures on BioResolve SCX mAb columns? - WKB65620
ENVIRONMENT
- BioResolve SCX mAb columns
- Strong Cation Exchange columns
ANSWER
SALT GRADIENTS:
| Pressuress obtained on a Waters Alliance HPLC using a salt gradient on BioResolve SCX mAb Columns | ||||
| Column Dimension | Flow Rate (mL/min) | System Pressure (psi) | Pressure with column (psi) | Column pressure (psi) |
| 4.6x50mm | 0.3 | 28 | 1185 | 1157 |
| 4.6x50mm | 0.4 | 30 | 1568 | 1538 |
| 4.6x50mm | 0.5 | 37 | 1950 | 1913 |
| 4.6x50mm | 0.6 | 45 | 2335 | 2290 |
| 4.6x50mm | 0.7 | 55 | 2733 | 2678 |
| 4.6x50mm | 0.8 | 64 | 3128 | 3064 |
| 4.6x50mm | 0.9 | 71 | 3541 | 3470 |
| 4.6x50mm | 1.0 | 79 | 3954 | 3875 |
| 4.6x50mm | 1.1 | 87 | 4375 | 4288 |
| 4.6x50mm | 1.2 | 96 | 4805 | 4709 |
| 4.6x100mm | 0.3 | 28 | 2558 | 2530 |
| 4.6x100mm | 0.4 | 30 | 3362 | 3332 |
| 4.6x100mm | 0.5 | 37 | 4147 | 4110 |
| 4.6x100mm | 0.6 | 45 | 4940 | 4895 |
|
Pressures Obtained on an ACQUITY UPLC H-Class Bio System using a slat gradient (constant MES Buffer pH with Increasing Salt Gradient. Highest pressure of the gradient is with 20mM MES 10mM Salt pH 7.0, column at 30° C) |
||||
|
Column Dimension |
Flow Rate (mL/min) |
System Pressure |
Pressure with column (psi) |
Column Pressure (psi) |
|
2.1x50mm |
0.1 |
407 |
1934 |
1527 |
|
2.1x50mm |
0.2 |
549 |
3560 |
3011 |
|
2.1x50mm |
0.3 |
695 |
5090 |
4395 |
|
2.1x50mm |
0.4 |
836 |
6734 |
5898 |
|
2.1x50mm |
0.5 |
976 |
8435 |
7459 |
|
2.1x50mm |
0.6 |
1117 |
10195 |
9078 |
|
2.1x50mm |
0.7 |
1255 |
12027 |
10772 |
|
2.1x50mm |
0.8 |
1397 |
13927 |
12530 |
|
2.1x100mm |
0.1 |
407 |
3674 |
3267 |
|
2.1x100mm |
0.2 |
549 |
6819 |
6270 |
|
2.1x100mm |
0.3 |
695 |
9850 |
9155 |
|
2.1x100mm |
0.4 |
836 |
13113 |
12277 |
pH GRADIENTS:
| Pressuress obtained on a Waters Alliance HPLC using a Waters' pH gradient with CX buffers and BioResolve SCX mAb Columns | ||||
| Column Dimension | Flow Rate (mL/min) | System Pressure (psi) | Pressure with column (psi) | Column pressure (psi) |
| 4.6x50mm | 0.3 | 25 | 1208 | 1183 |
| 4.6x50mm | 0.4 | 31 | 1610 | 1579 |
| 4.6x50mm | 0.5 | 39 | 2013 | 1974 |
| 4.6x50mm | 0.6 | 47 | 2420 | 2373 |
| 4.6x50mm | 0.7 | 51 | 2833 | 2782 |
| 4.6x50mm | 0.8 | 60 | 3256 | 3196 |
| 4.6x50mm | 0.9 | 68 | 3680 | 3612 |
| 4.6x50mm | 1.0 | 75 | 4108 | 4033 |
| 4.6x50mm | 1.1 | 83 | 4563 | 4480 |
| 4.6x100mm | 0.3 | 25 | 2583 | 2558 |
| 4.6x100mm | 0.4 | 31 | 3420 | 3389 |
| 4.6x100mm | 0.5 | 39 | 4252 | 4213 |
| 4.6x100mm | 0.6 | 47 | 5070 | 5023 |
|
Pressures Obtained on an ACQUITY UPLC H-Class Bio System WITH BioResolve CX-pH 10X Concentrates (pH Gradient). (The highest pressure is at about 30:70 A:B) |
||||
|
Column Dimension |
Flow Rate (mL/min) |
System Pressure |
Pressure with column (psi) |
Column Pressure (psi) |
|
2.1x50mm |
0.1 |
398 |
2080 |
1682 |
|
2.1x50mm |
0.2 |
540 |
3875 |
3335 |
|
2.1x50mm |
0.3 |
685 |
5579 |
4894 |
|
2.1x50mm |
0.4 |
820 |
7326 |
6506 |
|
2.1x50mm |
0.5 |
961 |
9081 |
8120 |
|
2.1x50mm |
0.6 |
1107 |
10867 |
9760 |
|
2.1x50mm |
0.7 |
1255 |
12660 |
11405 |
|
2.1x50mm |
0.8 |
1403 |
14620 |
13217 |
|
2.1x100mm |
0.1 |
398 |
3668 |
3270 |
|
2.1x100mm |
0.2 |
540 |
6842 |
6302 |
|
2.1x100mm |
0.3 |
685 |
9779 |
9094 |
|
2.1x100mm |
0.4 |
820 |
12515 |
11695 |
ADDITIONAL INFORMATION
BioResolve SCX Consumables Infographic
BioResolve SCX mAb Columns Care & Use Manual
mAb Charge Variant Standard Care & Use Manual
BioResolve CX pH Concentrates A and B Care & Use Manual
id65620, monoclonal antibody, UPBINARY