Can I shorten the run time of my 23 mer oligonucleotides if I switch from an ACQUITY UPLC Oligonucleotide C18, 1.7 µm, 2.1 mm x 50 mm column to an ACQUITY BEH300 C4 column? - WKB61505
Article number: 61505
ENVIRONMENT
- Oligonucleotide analysis
- Oligonucleotide separation technology (OST)
- Reverse phase
- ACQUITY OST C18
- ACQUITY BEH300 C4
ANSWER
Yes, but there will likely be less resolution, and method development would be required.
ADDITIONAL INFORMATION
Waters has not evaluated the use of 300A, C4 chemistries for ion pair reverse-phase analysis of oligonucleotides.
One method of decreasing run times for oligonucleotides of 23 mer on an ACQUITY UPLC Oligonucleotide C18, 1.7 µm, 2.1 mm x 50 mm column is to increase the flow rate by two times, decrease the gradient time by two, and increase temperature.
id61505, UPBINARY