What column is a good choice for metabolomics studies? - WKB55140
ENVIRONMENT
- UltraPerformance Liquid Chromatography (UPLC)
- Ultra High Performance Liquid Chromatography (UHPLC)
- High Performance Liquid Chromatography (HPLC)
- Column
- ACQUITY
- MaxPeak
- High Performance Surfaces (HPS)
- ACQUITY Premier
- Bridged Ethylene Hybrid (BEH)
- ACQUITY BEH Amide columns
- XBridge BEH Amide columns
- HILIC
- Metabolite identification
- Atlantis
- Z-HILIC
ANSWER
For UPLC:
- ACQUITY Premier BEH Amide, 130A, 1.7 µm Columns**
- ACQUITY UPLC BEH Amide, 130Å, 1.7µm columns
- Atlantis Premier BEH Z-HILIC, 95A, 1.7um Columns**
**MaxPeak HPS (High Performance Surfaces) column technology.
For HPLC:
- XBridge Premier BEH Amide Column, 130A, 2.5µm Columns**
- XBridge BEH Amide, 130A, 2.5um, 3.5um and 5um Columns.
- Atlantis Premier BEH Z-HILIC, 95A, 2.5um and 5um Columns**
**MaxPeak HPS (High Performance Surfaces) column technology.
ADDITIONAL INFORMATION
Because metabolomics studies look at polar metabolites, a HILIC (hydrophilic interaction chromatography) column is a good choice for this type of work. The columns listed above a HILIC packings. These are excellent choices for diverse polar compound retention, including both basic and acidic polar metabolites (as well as neutral polar analytes).
See the application note titled "Development of a Metabolomic Assay for the Analysis of Polar Metabolites Using HILIC UPLC/QTof MS" on pages 4-13 of the following application notebook:
Untargeted Metabolomics and Lipidomics Application Notebook
Also see:
Metabolomics & Lipidomics Website
MaxPeak Premier Columns Web Page
MaxPeak Premier Columns with MaxPeak High Performance Surfaces - Ordering Information
Improved Separation of RNA Nucleotides, Nucleosides, and Nucleobases on Atlantis Premier BEH Z-HILIC Columns (720007324)
Analysis of Sugar Alcohols and Allulose Using an Arc HPLC System with Refractive Index Detection (720007499, January 2022)
id55140, UPBINARY