What is the difference between ACQUITY UPLC BEH C18, ACQUITY UPLC Peptide BEH C18 and ACQUITY Premier Peptide BEH C18 columns?- WKB52711

ENVIRONMENT

• ACQUITY
• ACQUITY Premier
• MaxPeak
• High Performance Surfaces
• BEH C18
• Peptide BEH C18
• Column

ANSWER

All three designations refer to the same particle technology (bridged ethylene hybrid).  The Differences are as follows:

ACQUITY UPLC BEH C18: (130A)

• This type of column is packed in traditional stainless-steel hardware (frits and column body).
• The bulk packing used in this column is first QC tested with a small molecule test mix to ensure batch to batch reproducibility.

ACQUITY UPLC Peptide BEH C18 (130A and 300A)

• This type of column is packed in traditional stainless-steel hardware (frits and column body).
• The bulk packing used in this column is first QC tested with a peptide test mix (cytochrome C tryptic digest) to ensure batch to batch reproducibility for peptide separations.

ACQUITY Premier Peptide BEH C18 (130A and 300A)

• This type of column is packed in MaxPeak High Performance Surfaces (HPS) hardware.
  • Very inert column frits and column body.
  • Better peptide recovery out of the box.
• The bulk packing used in this column is first QC tested with a peptide test mix (cytochrome C tryptic digest) to ensure batch to batch reproducibility for peptide separations.

ADDITIONAL INFORMATION

Hybrid Particle Technology: Part 2: BEH: White Paper 

ACQUITY UPLC and ACQUITY Premier BEH Columns Care and Use Manual

ACQUITY UPLC and ACQUITY Premier Peptide BEH C18, 130 Å and 300 Å Columns Care and Use Manual

MaxPeak Premier | Non-Specific Adsorption – Infographic

ACQUITY Premier Columns for Peptide Analysis – Infographic 

Low Adsorption HPLC Columns Based on MaxPeak High Performance Surfaces – White Paper

For additional information on the Cytochrome C Digestion Standard (186006371), see the Cytochrome C Digestion Standard Care and Use Manual.