Can I analyze PEG (polyethylene glycol) or PEO (polyethylene oxide) with ACQUITY APC (advanced polymer chromatography) AQ columns? - WKB51590
ENVIRONMENT
- ACQUITY APC columns
- BEH SEC columns
- Polyethylene glycol
- Polyethylene oxide
ANSWER
No, this is not recommended. See additional information below.
ADDITIONAL INFORMATION
ACQUITY APC AQ columns are packed with an unbonded BEH (bridged ethylene hybrid) particle. PEG and PEO can undergo secondary interactions with this packing, so ACQUITY APC AQ columns are not the best choice for analysis of PEG or PEO.
Instead, Waters has found that the ACQUITY UPLC BEH SEC columns (normally used for protein size exclusion chromatography) work very well for PEG and PEO analysis. The packing in these columns is made with the BEH particle bonded with diol groups.
ACQUITY APC Columns Care & Use Manual
ACQUITY UPLC BEH SEC Columns and Standards Care & Use Manual
Advanced Polymer Chromatography - Method Development Tools for SEC Analysis of PEG
What is the difference between an ACQUITY APC AQ column and an ACQUITY BEH SEC column?