Can I analyze PEG (polyethylene glycol) or PEO (polyethylene oxide) with ACQUITY APC (advanced polymer chromatography) AQ columns? - WKB51590

ENVIRONMENT

• ACQUITY APC columns
• BEH SEC columns
• Polyethylene glycol
• Polyethylene oxide

ANSWER

No, this is not recommended. See additional information below.

ADDITIONAL INFORMATION

ACQUITY APC AQ columns are packed with an unbonded BEH (bridged ethylene hybrid) particle. PEG and PEO can undergo secondary interactions with this packing, so ACQUITY APC AQ columns are not the best choice for analysis of PEG or PEO.

Instead, Waters has found that the ACQUITY UPLC BEH SEC columns (normally used for protein size exclusion chromatography) will work for PEG and PEO analysis. The packing in these columns is made with the BEH particle bonded with diol groups.

ACQUITY APC Columns Care & Use Manual

ACQUITY UPLC BEH SEC Columns and Standards Care & Use Manual

Advanced Polymer Chromatography - Method Development Tools for SEC Analysis of PEG 

What is the difference between an ACQUITY APC AQ column and an ACQUITY BEH SEC column?

Hybrid Particle Technology: Part 2: BEH: White Paper