How do I connect the VanGuard Pre-column to an ACQUITY column? - WKB48031

Article number: 48031

OBJECTIVE or GOAL

Connect VanGuard Column Protection to an ACQUITY UPLC column and minimize any void space in the connection.

Carefully remove the black O-ring from the ferrule. (The ferrule is only lightly staked.).
- Do NOT pick up the VanGuard Pre-column by the compression screw after O-ring removal. Doing so, will cause the 2-piece ferule and the compression screw to disconnect from the tuning.
Hold the VanGuard Pre-column with the inlet side of the VanGuard on the bench top and the ferrule end pointing up, away from the bench top.
Bring the column inlet down over the ferrule end of the VanGuard Pre-column.
While the inlet of the column is facing down, the tighten the compression screw until it is finger-tight.
- Always maintain a little pressure on the VanGuard Pre-column during this process to make sure the tubing goes all the way into the column inlet end nut properly.
Flip the column/VanGuard stack so the VanGuard Cartridge is on top of the column.
- Use two wrenches to further tighten the VanGuard Pre-column to the inlet of the column.
  - Tighten the compression screw of the VanGuard Pre-column about 1/4 inch turn past finger-tight. You may need to tighten slightly more than 1/4 inch turn if a leak is present after 1.4 inch turn.
  - It is normal to see some threads.
  - Do not over tighten.
Remove the VanGuard Pre-column if you would like to check that the ferrule is seated properly.

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