What is the typical run time for XBridge BEH SEC columns? - WKB47495

ENVIRONMENT

• XBridge BEH 
• SEC
• run time
• empty column volume
• solvent front
• Size Exclusion Chromatography

ANSWER

For 7.8x150mm columns, if flowing at 0.86 mL/min, the typical run time is about 10 minutes.

For 7.8x300mm columns, if flowing at 0.86 mL/min, the typical run time is about 20 minutes.

ADDITIONAL INFORMATION

Total run time is based on column volume and flow rate.

The empty column volume for a 7.8x150mm column is about 7 mL. The solvent front should elute a 7.8x150mm column at about 8.14 min if flowing at 0.86 mL/min.

The empty column volume for a 7.8x300mm column is about 14 mL.The solvent front should elute a 7.8x300mm column at about 16.28 min if flowing at 0.86 mL/min.

XBridge Protein BEH SEC Columns & Standards Care & Use Manual (lit code 720005206en) and

Successful Transfer of Size-Exclusion Separations between HPLC and UPLC (lit code 720005214EN)