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What is the typical run time for XBridge BEH SEC columns? - WKB47495

Article number: 47495

ENVIRONMENT

  • XBridge BEH SEC
  • SEC

ANSWER

For 7.8x150mm columns, if flowing at 0.86 mL/min, the typical run time is about 10 minutes.

For 7.8x300mm columns, if flowing at 0.86 mL/min, the typical run time is about 20 minutes.

ADDITIONAL INFORMATION

Total run time is based on column volume and flow rate.

The empty column volume for a 7.8x150mm column is about 7 mL. The solvent front should elute a 7.8x150mm column at about 8.14 min if flowing at 0.86 mL/min.

The empty column volume for a 7.8x300mm column is about 14 mL.The solvent front should elute a 7.8x300mm column at about 16.28 min if flowing at 0.86 mL/min.

XBridge Protein BEH SEC Columns & Standards Care & Use Manual (lit code 720005206en): http://www.waters.com/waters/support.htm?lid=134819782&type=USRM

Successful Transfer of Size-Exclusion Separations between HPLC and UPLC (lit code 720005214EN): http://www.waters.com/waters/library.htm?cid=511436&lid=134822077&locale=101

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