What is the highest proportion of organic that a BEH SEC column can be exposed to in the mobile phase? - WKB47450
Article number: 47450
ENVIRONMENT
- BEH SEC column
- Organic mobile phase component
ANSWER
The care and use manual recommends using less than 20% organic.
ADDITIONAL INFORMATION
The BEH particle can withstand higher amounts of organic; however, at higher concentrations, precipitation can become a problem with some biomolecules. Also, repeated exposure to high organic may result in a chromatography change if the column is run under high aqueous again.
For additional information, see the ACQUITY UPLC Protein BEH SEC Columns and Standards Care and Use Manual and the XBridge Protein BEH SEC Columns and Standards Care and Use Manual.
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