How do I determine the best column equivalent when moving from HPLC to UPLC, keeping the efficiency the same? - WKB32689
Article number: 32689
OBJECTIVE or GOAL
Determine the UPLC column closest in efficiency to the HPLC column.
ENVIRONMENT
- UPLC
- HPLC
- XBridge
- ACQUITY UPLC
- CORTECS UPLC Columns
- scale
- efficiency
- plate count
- equivalent performance
- transfer
- L/dp
- 1.6um
- 1.7um
- 1.8um
- 2.5um
- 2.7um
- 3.0um
PROCEDURE
- Use the HPLC column dimension as the starting point. Example: 3.5µm, 4.6 x 150mm
- Determine the L/dp ratio, where L = column length and dp = particle size.
- The L/dp ratio is 150/3.5 = 42.
- Find the column chemistry closest to the original HPLC column chemistry. ACQUITY UPLC particle sizes are 1.6, 1.7, or 1.8 µm, depending on the brand.
- The L/dp ratio of 75/1.7 = 44.
ADDITIONAL INFORMATION
For additional information, see The Promise of Small Particles.
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