Can I use BEH SEC columns in high-organic conditions? - WKB30717

ENVIRONMENT

• Size exclusion chromatography (SEC)
• Column capabilities
• Bridged ethylene hybrid (BEH)
• XBridge
• ACQUITY

ANSWER

Yes, the particle itself can handle being run under higher organic conditions. However, Waters cautions in the care and use manual that protein precipitation becomes a critical issue with higher than 20% organic conditions. In addition, with ongoing exposure to greater than 10% organic conditions, the column will have a greater possibility of not returning to original chromatographic conditions.  

ADDITIONAL INFORMATION

ACQUITY UPLC BEH SEC Columns and Standards Care & Use Manual

XBridge Protein BEH SEC Columns & Standards Care & Use Manual