What is a good column choice for the analysis of water-soluble vitamins? - WKB28185

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Article number: 28185

ENVIRONMENT

Water-soluble vitamins
Polar compound retention
Ascorbic Acid
Nicotinamide
Pyridoxal
Riboflavin
Nicotinic acid
Thiamine
Ascorbic acid
B12
Folic Acid
Vitamin C
Atlantis Premier BEH C18 AX
MaxPeak
High Performance Surfaces (HPS)
High Strength SIlica (HSS)
T3
Bridged Ethylene Hybrid (BEH)
High Strength Silica (HSS)
Column
CORTECS
Hydrophilic Interaction Chromatography (HILIC)

ANSWER

Mixed-Mode: (Reversed-Phase with Weak Anion Exchange)

Atlantis Premier BEH C18 AX (1.7um, 2.5um and 5um)¹

Reversed-Phase:

ACQUITY Premier HSS T3 (1.8um)²
ACQUITY UPLC HSS T3 (1.8um)
CORTECS Premier T3 (1.6um & 2.7um, solid core particles)¹
CORTECS T3 (1.6um & 2.7um, solid core particles)
XSelect Premier HSS T3 (2.5um)¹
XSelect HSS T3 (2.5um, 3.5um, 5um & 10um)
Atlantis T3 (3um, 5um & 10um)
Atlantis dC18 (3um, 5um & 10um)

HILIC: (Hydrophilic Interaction Chromatography)

Atlantis Premier BEH Z-HILIC (1.7um, 2.5um and 5um)¹
ACQUITY Premier BEH Amide (1.7um)²
ACQUITY UPLC BEH Amide (1.7um)
XBridge Premier BEH Amide (2.5um)¹
XBridge BEH Amide (2.5um, 3.5um, 5um & 10um)

1. Packed in MaxPeak Premier hardware in 2.1mm I.D. and 4.6mm I.D.

2. Packed in MaxPeak Premier hardware in 2.1mm I.D.

MaxPeak HPS (High Performance Surfaces) makes the stainless steel frits and column surfaces inert so that acidic analytes will not adsorb to the positive charges of the stainless steel as samples contact the stainless steel surfaces inside the column.