How do the normal-phase column chemistries Cyano, Amide, and Silica rank in order of polarity? - WKB259955
Article number: 259955
ENVIRONMENT
- Normal phase
- Column chemistry
- Polar retention
- Cyano (CN or Nitrile)
- Amide
- Silica
ANSWER
Silica is the most polar, with Cyano and Amide roughly equal in polarity.
ADDITIONAL INFORMATION
Although Cyano and Amide are more equal in their polar retention, they will confer very different selectivity in normal-phase chromatography.
For additional information, see Will XBridge BEH Amide retain my sugars?
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