How do I choose a UPLC or HPLC column chemistry for a method? - WKB256525
Article number: 256525
ENVIRONMENT
- Method development
- Column chemistry
- Screening
- UPLC
- HPLC
- UHPLC
ANSWER
See a sample strategy for choosing a column chemistry in the application note Using a Systematic Screening Protocol and MaxPeak HPS Technology to develop a UHPLC Method for the Analysis of Deferoxamine and its Forced Degradation Products.
ADDITIONAL INFORMATION
Considerations for choosing a column chemistry might also include:
- Is the sample a water-soluble compound or a very nonpolar compound? In what is the sample soluble?
- Is the sample ionizable? What is the pKa if known?
- Is the sample metal-sensitive?
- Is the sample more stable at high pH, or does it give a higher response in mobile phase run at high pH?
- What is the molecular weight of the sample?
For additional information, see The Column Coach.