What is the advantage of using the CSH C18 column chemistry instead of the BEH C18 column chemistry? - WKB250290

ENVIRONMENT

• Column characteristics
• Reverse phase
• Charged surface hybrid (CSH)
• Bridged ethylene hybrid (BEH)
• C18

ANSWER

Under low ionic strength, buffered mobile phases*, the CSH C18 provides better peak shape for basic compounds and increased loading capacity compared with the BEH C18 chemistry run in these types of mobile phases.

The CSH particle also exhibits more rapid column equilibration after changing the mobile phase pH.

* Mobile phases that contain formic acid and acetic acid

ADDITIONAL INFORMATION

For additional information, see Increasing Peak Capacity in Reversed-Phase Peptide Separation with Charged Surface Hybrid (CSH) C18 Columns

and Practical Applications of Charged Surface Hybrid (CSH) Technology.