What are some suggestions for optimizing peak capacity in nano LC separations? - WKB237327

ENVIRONMENT

• nanoEase M/Z
• ACQUITY M-Class
• nanoACQUITY
• Peptide separation
• Method development
• Peak capacity
• nano LC-MS
• Trap column

ANSWER

Suggestions for optimizing peak capacity include the following:

• Investigate the use of the CSH C18 chemistry for high peptide peak capacities in the presence of formic acid mobile phases.
• Investigate the time and flow rate to load the sample on the Trap column if a Trap column is being used. An example of Trap flow loading is 5 to 25 uL/min for 1-3 minutes.
• Investigate the use of differential column heating on the peptide separation.

ADDITIONAL INFORMATION

For additional information, see Optimizing Peak Capacity in Nanoscale Trap-Elute Peptide Separations with Differential Column Heating

and Considerations for Selecting the Optimal Stationary Phases for Proteomic Trap-and-Elute Nanochromatography.