How much sample can I load on a 3.0 x 100 mm reverse-phase column? - WKB236864
Article number: 236864
ENVIRONMENT
- Reverse-phase columns
- C18
- Mass load
ANSWER
The approximate load for a small molecule is 2.5 mg. The approximate load for a small, water soluble peptide is 0.25–0.5 mg.
ADDITIONAL INFORMATION
The mass load varies based on sample solubility in water and small molecule vs. large molecule. It is determined experimentally.
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