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Why do I see bubbles leaving my ACQUITY UPLC column when I try to install it? - WKB236372

Article number: 236372

ENVIRONMENT

  • ACQUITY UPLC
  • Column installation
  • Air bubbles

ANSWER

Possible causes include:

  • Improper system priming before column installation
  • Excess dissolved gases in mobile phase prior to column installation
  • Degasser on system not working
  • Poor column connection at inlet of column (worn fittings, improper connection, etc.)

ADDITIONAL INFORMATION

 

id236372, eluent, UPBINARY

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