Can I perform a non-aqueous reverse-phase method (no water)? - WKB232654

ENVIRONMENT

• Method development
• Reverse-phase
• Non-aqueous separations

ANSWER

Yes

ADDITIONAL INFORMATION

Water is not required to perform a reverse-phase method if an alternative polar solvent can be used that does not lead to the elution of the analytes of interest upon injection. 

There are examples of non-aqueous reverse-phase separations in the literature.  

Reverse-phase essentially means that the stationary phase is non-polar and analytes are eluted with a non-polar solvent. 

An examnple application is:  Non-aqueous reverse phase purification of carotenes using a small particle preparative packing

In this application,