What is a good UPLC column choice for analysis of water-soluble cationic polymers? - WKB232422
ENVIRONMENT
- UltraPerformance Liquid Chromatography (UPLC)
- Size exclusion chromatography (SEC)
- Bridged ethylene hybrid (BEH)
- Water-soluble polymers
- ACQUITY
ANSWER
ACQUITY UPLC Protein BEH SEC Columns
ADDITIONAL INFORMATION
Although Waters offers ACQUITY APC AQ columns, the particles in those columns exhibit a high degree of negative surface charges because they are unbonded BEH particles.
ACQUITY UPLC Protein BEH SEC columns feature a diol-bonded BEH particle that greatly reduces the number negative silanol surface charges. Choice of pore size is based on the hydrodynamic radius (size in solution) of the polymer.
In addition, the newer ACQUITY Premier Protein SEC 250A columns feature a very hydrophilic PEO (polyethylene oxide) bonded phase on a BEH particle. This is extremely hydrophilic and may or may not be a great choice for very hydrophilic polymers. This very hydrophilic bonded phase may lead to non-specific adsorption of hydrophilic polymers to a much higher degree than the diol-bonded phase in BEH SEC columns.
See:
ACQUITY UPLC SEC Product Solution Brochure
XBridge Protein BEH SEC Columns Brochure
BEH SEC Column Technology – Infographic
ACQUITY UPLC BEH SEC Columns and Standards Care & Use Manual
XBridge Protein BEH SEC Columns & Standards Care & Use Manual
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