What column should I try if a lipid-conjugated peptide does not elute from a C18 or a C8 column? - WKB231427

ENVIRONMENT

• Column alternatives
• Lipid-conjugated peptide

ANSWER

Options to try might include:

ACQUITY Premier Protein BEH C4, 300Å

ACQUITY UPLC BEH C4, 300Å

XBridge Premier Protein BEH C4, 300Å

XBridge Protein BEH C4, 300Å 

ACQUITY UPLC BEH Phenyl

XBridge BEH Phenyl

Delta-Pak C4, 100Å

Delta-Pak C4, 300Å

ADDITIONAL INFORMATION

Use of a shorter alkyl chain such as a C4 will be less retentive compared to C18 and C8. A wider, 300Å pore may result in some loss in resolution vs. a 130Å pore size.

Use of a phenyl bonded phase may result in retention similar to that of a C8 with alternate selectivity.

Only experimentation will confirm retentivity and selectivity of a given analyte on various stationary phases.