What is a good UPLC, UHPLC, or UPLC column choice for analysis of up to 160mer protected (trityl-on) single-stranded DNA oligonucleotides that have cyanoethylether hydrophobic protection groups on each phosphodiester bond? - WKB231138
ENVIRONMENT
- Column suitability
- Oligonucleotide analysis
- Trityl-on
- Hydrophobic
- DNA
ANSWER
ACQUITY Premier Peptide BEH C18, 300A, 1.7µm columns
ACQUITY UPLC Peptide BEH C18, 300A, 1.7µm columns
XBridge Premier Peptide BEH C18, 300A, 2.5µm columns
XBridge Peptide BEH C18, 300A, 2.5um XP or 3.5µm columns
ADDITIONAL INFORMATION
Waters has published no methods on neutral, hydrophobic oligonucleotide analysis.
Waters has published no method for the analysis of oligonucleotides up to 160mer using a reverse-phase column, but a 300A pore size column might be considered for this separation. Ion pairing is not needed because this oligonucleotide type is neutral. A simple reverse-phase gradient can be used.
Experimentation is needed to determine if a resolution of n-1 can be achieved.
Related links:
MaxPeak Premier | Non-Specific Adsorption – Infographic
ACQUITY Premier Columns for Peptide Analysis – Infographic
ACQUITY UPLC and ACQUITY PREMIER Peptide BEH C18, 130 Å and 300 Å Columns Care and Use Manual
XBridge Peptide BEH C18 130A and 300A Columns Care and Use