What is a good, general purpose, reverse-phase column for lab-scale purification of small nonpolar molecules? - WKB230355
ENVIRONMENT
- Lab-scale purification
- Small nonpolar molecules
- Prep columns
- Bridged ethylene hybrid (BEH)
- Charged surface hybrid (CSH)
- XBridge
- XSelect
ANSWER
XBridge BEH C18
XSelect CSH C18
ADDITIONAL INFORMATION
XBridge BEH C18 and XSelect CSH C18 are great choices to purify small, nonpolar analytes.
If you plan to perform repeated pH (high pH, low pH) switching on the same column, XSelect CSH is a better option because this packing re-equilibrates faster, to pH adjustments, so that peak shape for basic analytes remains sharp when moving from high pH back to low pH.
Also see:
Preparative (Purification) HPLC Columns Web Page
OBD Frequently Asked Questions
On-line Preparative Columns Calculator
Preparative OBD Columns Wall Chart
Waters OBD Prep Column Technology White Paper
Hybrid Particle Technology: Part 2: BEH: White Paper
Hybrid Particle Technology: Part 3: CSH: White Paper
At-Column Dilution Application Notes
Developing Focused Gradients for Isolation and Purification
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