Why am I getting sample peaks eluting after the place where the uracil peak elutes on my BEH SEC column? - WKB230136
Article number: 230136
ENVIRONMENT
- Size exclusion chromatography (SEC)
- Protein analysis
- XBridge Premier BEH
- ACQUITY Premier BEH
- XBridge BEH SEC
- ACQUITY BEH SEC
ANSWER
Sample peaks eluting after the Vt marker (uracil) indicate that these peaks are retained on the sorbent in a retentive manner.
ADDITIONAL INFORMATION
If using XBridge Premier BEH 250 SEC or ACQUITY Premier BEH 250 SEC columns, see Waters ACQUITY and XBridge Premier Protein SEC 250A Columns: A New Benchmark in Inert SEC Column Design.
If using standard XBridge BEH SEC or ACQUITY BEH SEC columns, see Method Development for SEC of Monoclonal Antibodies and Higher Order Aggregates.
id230136,