What is a good UPLC, UHPLC, and HPLC column choice for analysis and purification of small polar molecules that contain phosphate groups? - WKB229862

ENVIRONMENT

• Column selection
• UltraPerformance Liquid Chromatography (UPLC)
• High Performance Surfaces (HPS)
• Bridged ethylene hybrid (BEH)
• ACQUITY Premier BEH Amide
• XBridge Premier BEH Amide
• MaxPeak Premier

ANSWER

UPLC:

• ACQUITY Premier BEH Amide, 1.7µm  (first choice)
• ACQUITY BEH Amide, 1.7µm

UHPLC:

• XBridge Premier BEH Amide, 2.5µm (first choice)
• XBridge BEH Amide, 2.5µm

HPLC (analytical and prep):

• XBridge BEH Amide, 2.5µm, 3.5µm, and 5.0µm

ADDITIONAL INFORMATION

The BEH Amide packing is a great choice for the retention of polar small molecules that contain phosphates.

For analytical separations, ACQUITY Premier and XBridge Premier BEH Amide columns are recommended because they use MaxPeak HPS (High Performance Surfaces) hardware. MaxPeak Premier columns prevent analyte adsorption to the stainless steel surfaces inside these columns, whereas traditional stainless steel does not prevent adsorption.

MaxPeak Premier Columns Web Page

Low Adsorption HPLC Columns Based on MaxPeak High Performance Surfaces – White Paper

MaxPeak Premier Columns with MaxPeak High Performance Surfaces - Ordering Information

ACQUITY UPLC and ACQUITY Premier BEH Columns Care and Use Manual

XBridge XP and XBridge PREMIER 2.5 μm BEH Columns Care and Use Manual

XBridge Columns Care & Use Manual

Preparative (Purification) HPLC Columns Web Page

On-line Preparative Columns Calculator

Waters OBD Prep Column Technology White Paper