My retention times shift from one Styragel GPC column bank to another. Is this a problem? - WKB229348
Article number: 229348
ENVIRONMENT
- Styragel
ANSWER
No, for GPC you cannot compare retention times from one column (bank) to another. It is not like reverse phase. That is why you need to benchmark each bank separately. If you are obtaining good column efficiency, you should go with this. Recalibrate and evaluate the samples run on the new column against the new curve and distributions generated on the new bank.
ADDITIONAL INFORMATION
id229348, EMPGPC, GPCV2K