What is a good UPLC column choice for LC-MS/MS analysis of nicotinamide (vitamin B3)? - WKB228172

ENVIRONMENT

• Ultra High Performance Liquid Chromatography (UHPLC)
• Bridged ethylene hybrid (BEH)
• Polar compound retention
• Reversed phase
• Mixed mode
• Anion exchange
• MaxPeak
• High Performance Surfaces (HPS)
• Premier
• Atlantis
• BEH C18 AX
• BEH C18
• CSH C18
• HSS T3

ANSWER

Atlantis Premier BEH C18 AX

ACQUITY Premier BEH C18

ACQUITY Premier CSH C18

ACQUITY Premier HSS T3

ACQUITY UPLC BEH C18

ACQUITY UPLC CSH C18

ACQUITY UPLC HSS T3

ADDITIONAL INFORMATION

See Enhancing the LC-MS/MS Analysis of B-group Vitamins with MaxPeak High Performance Surfaces Technology (lit code 720007264, May 2021)

• This application note includes the analyte nicotinamide. It shows performance of the following system and column in a pH 5.0 mobile phase: 
  • ACQUITY Premier System
  • Xevo TQ-S micro MS
  • ACQUITY Premier BEH C18 Column

See related journal article: Characterization of a highly stable mixed-mode reversed-phase/weak anion-exchange stationary phase based on hybrid organic/inorganic particles

• This article was written by Waters. It compares several analytes, including nicotinamide. It compares the following columns in a pH 3.0 mobile phase:
  • Atlantis Premier BEH C18 AX
  • ACQUITY UPLC BEH C18
  • ACQUITY UPLC CSH C18
  • ACQUITY UPLC HSS T3

Related:

MaxPeak Premier Columns Web Page

ACQUITY UPLC and ACQUITY Premier Columns Web Page

Atlantis Premier Columns Web Page

MaxPeak Premier Columns with MaxPeak High Performance Surfaces - Ordering Information