What is the difference between the Symmetry C18 100Å, 5 µm, 3.9 mm X 150 mm column (WAT046980) and the Symmetry C18 300Å, 5 µm, 3.9 mm X 150 mm column (WAT106154)? - WKB226913

ENVIRONMENT

• High Performance Liquid Chromatography (HPLC)
• Column comparison
• Symmetry

ANSWER

WAT046980 has 100Å pores.

WAT106154 has 300Å pores.

ADDITIONAL INFORMATION

• 300Å Symmetry C18 columns may show decreased retention times and, in some cases, decreased resolution compared with 100Å Symmetry C18 columns of the same particle size and column dimension.
• 300Å Symmetry C18 columns may not require as many empty column volumes to re-equilibrate compared with 100Å Symmetry C18 columns of the same particle size and column dimension. This would need to be determined experimentally.