Is column cleaning efficiency higher when the temperature of the column oven is higher? - WKB223985

ENVIRONMENT

• UPLC/HPLC columns

ANSWER

Generally, the cleaning effect is stronger when the column temperature is higher.

However, be sure to check the usable temperature and pH range according to the column maintenance manual before proceeding.

The upper limit of the column temperature that can be used depends on the pH of the mobile phase. Check the pH of the mobile phase and cleaning solution before cleaning.

In addition, column life may be impaired if analysis or cleaning is performed under conditions close to the upper limit of temperature.

It is most effective to implement an appropriate sample pretreatment method to remove contaminants adsorbed on the column.

It is also important to use pre-column filters and guard columns to minimize the buildup of contaminants on the analytical columns.

ADDITIONAL INFORMATION