Can I inject samples that contain 20% ethylene glycol and 20mM sodium caprylate onto the XBridge Protein BEH C4 300Å, 3.5 µm, 4.6 mm X 250 mm, 10K - 500K column (186004505)? - WKB223670

ENVIRONMENT

• High Performance Liquid Chromatography (HPLC)
• Bridged ethylene hybrid (BEH)
• Ethylene glycol
• Sodium caprylate
• XBridge Protein BEH C4

ANSWER

Inject samples with caution because ethylene glycol and sodium caprylate may build up on the column and cause high pressure and/or peak shape problems.

Use of a guard cartridge to protect this column is recommended.

ADDITIONAL INFORMATION

Ethylene glycol is very viscous and can build up on your column.

Sodium caprylate is very fatty and can also build up on your column.

The recommended guard cartridge and holder to protect part 186004505 are:

• 186007235 - XBridge Protein BEH C4 Sentry Guard Cartridge, 300Å, 3.5 µm, 4.6 mm X 20 mm, 1K - 30K, 2/pk

• WAT046910 - Holder Kit for Cartridge and Guard Columns

See the Sentry Guard Columns Care & Use Manual for information about this guard holder.