What reverse-phase column is recommended for analysis of a PEGylated small molecule? - WKB207472
Article number: 207472
ENVIRONMENT
- Method development
- Small molecule analysis
- Reverse-phase columns
- CORTECS
- XBridge
ANSWER
Reverse-phase column options to explore for PEGylated small molecules are CORTECS C8 or C18 2.7 µm and for higher PEG molecular weight try XBridge BEH300 C18 or XBridge BEH300 C4.
ADDITIONAL INFORMATION
Higher PEG molecular weight (>2K) may require hotter column temperatures to achieve good results.
For additional information, see PEGylated Protein Analysis by Size-Exclusion and Reverse-Phase UPLC.
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