Will sodium chloride shorten the lifetime of a Sugar-Pak 10 µm, 6.5 mm X 300 mm column (WAT085188)? - WKB196685

ENVIRONMENT

• High Performance Liquid Chromatography (HPLC)
• Sugar analysis
• Column care and use

ANSWER

No, but see the "Additional Information" section.

ADDITIONAL INFORMATION

The recommended mobile phase for this column is deionized, bacteria-free water containing approximately 0.0001 M calcium EDTA (50 mg/L). Do not use sodium chloride in the mobile phase. Use of sodium chloride in the mobile phase will require column regeneration as per the care and use manual because calcium ions may be disassociated from the particle surfaces in the presence of other cations.

Salts and organic acids present in crude samples can co-elute with sugars and interfere with the analysis. Typically, salts of strong acids such as sodium chloride elute near the void volume. Salts of weak acids, such as lactate and acetate, elute later.  

See the Sugar Pak I Column Care & Use Manual for additional guidance on the use of this column.