What causes poor recovery of sialylated glycans on my Glycan BEH Amide column? - WKB194277
ENVIRONMENT
- Bridged ethylene hybrid (BEH)
- Sialylated glycans
- Glycan BEH Amide
ANSWER
Adsorption to stainless steel surfaces
ADDITIONAL INFORMATION
To lower the amount of adsorption of sialylated glycans to stainless steel surfaces, you can perform a Glycan BEH Amide Column - LC system passivation with Fetuin - protocol.docx.
Find additional column conditioning instructions in the following care and use manuals:
- ACQUITY UPLC Glycan BEH Amide, 130 Å, 1.7 μm Columns, ACQUITY PREMIER Glycan BEH Amide, 130 Å, 1.7 μm Columns, and Glycan Performance Test Standards
- XBridge Glycan BEH Amide, 2.5 µm XP, 130Å, and 3.5 µm Columns and Standards Care and Use Manual
To address the issues with sialylated glycan adsorption to stainless steel surfaces, Waters has introduced the following columns:
- ACQUITY PREMIER Glycan BEH Amide
- ACQUITY PREMIER Glycan BEH C18 AX
These columns include MaxPeak HIgh Performance Surfaces, which prevent adsorption of analytes to stainless steel surfaces.
See:
ACQUITY PREMIER C18 AX Charge-Based Glycan Solution Infographic
Low Adsorption HPLC Columns Based on MaxPeak High Performance Surfaces - White Paper
PREMIER Columns with MaxPeak High Performance Surfaces - Ordering Information
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